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'i§wÏ7Yså¿Ôó(A¡é+ñ[qÉ¶EOŠ©­B{èw Ú ç ’!ë"›$‹%# ¨	·	UmsŒ“ÌC°ÀJ€¶OÇ8j¦½IÐŸà—U!ì!s"¼"´$h%  c´r5}-¢Z�©ã%š;U;ŠIÊñøÿ‹   O | ¡"z$•$?%> [¤Q"×z¤§Ù-aÍÐ Ñòµ[½†°h.JŒ«@/258{šâX¿V�“œŸ¢bs—èú � K!R!÷"Q#j$ ù`k�Nõ‹K
 &§¸ögÞÆš5!2"�# € p•¢öÀî-ü¡©KW&!Y"  L…A•»ˆ8K×ÂùKÏ¯ ”"¥"l# _=t„ï
Uså„œÀíF¦ófò'b¥Ä!*!0!ã!Ó"´#% ºwºÃæ2ÄÆ	Í	Ü	ù	B
]
v·ù�%)•¨êMrskâ¶ !z!m"Ô$ ß 1îfW
Ñc‹¬sºÇ3�çb)r £ Ð ƒ"ã"v$8%/  Q o,e©}¯	j	{	é	†Þê¶üÞÚ-{C[dÑé½ý)läz% > ×!Þ!9"W#�$%v%|%(  €>Ìò&jÃ0	%
§
%‚Ž¦ Oæ3£’ðó³>Tî‘&9ÎÔ- c!,"Û#'$%m% ðH,vrïY	d	Ch?hH”]ŒØäíóÊ#[%7 . ¹ 4RÚ%	

<<IÆ2;¶ûeoT”¦Èû �’Úû�
Pˆ4[oÜ°B I Y Ë!ò!"˜"#X$¥$%/%Ò%  ~§‰dD=ÅcO%»ØÚÜçoÌûP!x!t$Ò$-%, 1…©®»C¡&:	Ù	¼
LÊÍ36Þ«³`çSØQ“£æ@"’�ïý) p À"#a#å#$$/$/ È£°µÎã£î\žÇF	¹7Î6U|Ôà [)¿DYrw„0)R\jÖ, ¨ Â €!#6#y#ç#Ù$! r Í ô ¯ÉÍ3í<d
fo‡8G
NWå‡Ç’6HÆ – ?#1$Ê$" H‹ªDZ�{ 	*	M	¾
½­d+÷\ö´Eñ0>feËh� Ä"†$; d ),:ŠŸ«Á(xšQTõx‡Àé
 cilz�vËk¥9?Ú*^ap�–±èOvr´wÑs� ! š �!#]#J$ï$<%©%. �¾ÉãqZ{¨Q	Â	û
Ä­¬ÜTã‹µæCb-Ïy³û
~“VaÚ[!ñ"#.#2#g#’#I%‚% Å 3hÒNü¤�nP|éÄKPuÕV”@¨r ½ }"î#ª$d% hKR›�(
p
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#òwÁso·­ÝÂÚ†²"ç"c#®#$‚$( | \ #EKÄÏ9		<	£	m
È
�˜áˆ‹štvwl�5þ(× ä Œ!#ô$X%. !+¡Ò>Cq£,`l‹Ì*4ÐT¡ï¦Æi™¥Hz :‰µÏ¨þAŠ–çÿ
  »"P#  o¡jsôî
T›¿¦eñõa ™¨J%!1"“"(  Þ e+¹Â%i	Û	è	$�b«2ÝïÝSÙézBc%jÐ8kã$ , ¢ b!Ö!8"&$# }ï„B¦+q$	X	

;Ì25Ý:µ_nS’¢‘æŽ‡×ò`#.$6 q ®(‰¢ÀÈâ©Ì2™ì†�Æbn†F¬¸cuö[~ä]•¾ð/=CNƒeq5GÊÕvÅŒ • Á Ã"Ø$! [JÈgpYÃ	¢	ú
Ãà‡™ÛSOåJu¶UÜú?ð"
#í#
$W%c%& Ý n iƒ¸$ŠËç	í
#:ÏS1¥îdè�yå$9iŸ˜´Î•æñ	 + A p ¬­abcdefno‡ˆ½¾¿ÇË#…¡	ì
9am…Eab†˜GHI}µx½ï÷øù<M‚–—É’“”äå‰ Š ‹ Â"ï"$	$  8V  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                        C                                                                                                                                                                                                                                                                                                              L                                                    
   	                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                	                                                                                    4            
               /                        	                                                                 ?                                                            	                      +                                
   	                              	               	            8         
            "               g   .                              	                                                
      
         /         
         2   ;   7      <   F      &   ;   /   '   Ë   f      ,   *   #   B   *   Ž                     C                                                                                                                                            L                        
 	                                                                                                                                                                                                                                                                            	                              4    
     /        	                       ?                    	        +            
 	          	     	    8   
    "     g .          	                
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   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  d<Notes>
<UUID>745e6234-f4b5-46df-b965-d6e22e19416b</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="1:52:11">2024.4.24</Created>
<LastModified UTC="2:0:5">2024.4.24</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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ÎŸ   w<AlignableSequences><Sequence name="pCDH-CMV-MCS-EF1-Blast  (2778 .. 3506)" ID="0" sortOrder="1"/></AlignableSequences>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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</Notes>
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<Type>Synthetic</Type>
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<LastModified UTC="1:58:28">2024.4.24</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7759-7780" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7666-7682" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7690-7706" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; 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This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="BSD" directionality="1" translationMW="13668.05" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="5746-6144" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Aspergillus terreus BSD&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to blasticidin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;blasticidin S deaminase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGVNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIKAIVKDSDGQPTAVGIRELLPSGYVWEG*"/></Q></Feature><Feature recentID="18" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5740-5749" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature></Features>
  ¢<?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="mCherry-R" sequence="TTGGTCACCTTCAGCTTGG" description="mCherry, reverse primer"><BindingSite location="2819-2837" boundStrand="1" annealedBases="TTGGTCACCTTCAGCTTGG" meltingTemperature="57"><Component hybridizedRange="2819-2837" bases="TTGGTCACCTTCAGCTTGG"/></BindingSite><BindingSite simplified="1" location="2819-2837" boundStrand="1" annealedBases="TTGGTCACCTTCAGCTTGG" meltingTemperature="57"><Component hybridizedRange="2819-2837" bases="TTGGTCACCTTCAGCTTGG"/></BindingSite></Primer><Primer recentID="1" name="mCherry-F" sequence="CCCCGTAATGCAGAAGAAGA" description="mCherry, forward primer"><BindingSite location="3087-3106" boundStrand="0" annealedBases="CCCCGTAATGCAGAAGAAGA" meltingTemperature="54"><Component hybridizedRange="3087-3106" bases="CCCCGTAATGCAGAAGAAGA"/></BindingSite><BindingSite simplified="1" location="3087-3106" boundStrand="0" annealedBases="CCCCGTAATGCAGAAGAAGA" meltingTemperature="54"><Component hybridizedRange="3087-3106" bases="CCCCGTAATGCAGAAGAAGA"/></BindingSite></Primer></Primers>
  Ü<Notes>
<UUID>745e6234-f4b5-46df-b965-d6e22e19416b</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>PiggyBac-EF1a-mCherry-LMNB1(human)-EF1a-Blast</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="1:52:11">2024.4.24</Created>
<LastModified UTC="3:2:56">2025.7.1</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y     K         
?????                                                                                                       ÿ d      DO ÿþ              E              ÿÿÿÿYô? P                                                                                                                                                             <StrandColors><TopStrand><ColorRange range="0..2665" colors="black"/><ColorRange range="2666..3412" colors="green"/><ColorRange range="3413..3836" colors="purple"/><ColorRange range="3837..3837" colors="red"/><ColorRange range="3838..5172" colors="purple"/><ColorRange range="5173..9686" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..2665" colors="black"/><ColorRange range="2666..3412" colors="green"/><ColorRange range="3413..3836" colors="purple"/><ColorRange range="3837..3837" colors="red"/><ColorRange range="3838..5172" colors="purple"/><ColorRange range="5173..9686" colors="black"/></BottomStrand></StrandColors>   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
